A 1217, 191–198 (2010b)Ĭooper, R.S., Liberman, D.A.: Fixed-bed adsorption kinetics with pore diffusion control. USA 107, 16811–16826 (2010a)Ĭhung, W.K., Hou, Y., Holstein, M., Freed, A., Makhatadze, G.I., Cramer, S.M.: Investigation of protein binding affinity in multimodal chromatographic systems using a homologous protein library. 28, 1252–1264 (2005)Ĭhung, W.K., Freed, A.S., Holstein, M., McCallum, S., Cramer, S.M.: Evaluation of protein adsorption and preferred binding regions in multimodal chromatography using NMR. 49, 3066–3073 (2003)Ĭarta, G., Ubiera, A.R., Pabst, T.M.: Protein mass transfer kinetics in ion exchange media: measurements and interpretations. Wiley, New York (1960)Ĭarta, G., Ubiera, A.R.: Particle-size distribution effects in batch adsorption. As a result, for the mAb, much higher column dynamic binding capacities are obtained for Nuvia cPrime than for Capto MMC.īird, R.B., Stewart, W.E., Lightfoot, E.N.: Transport Phenomena. Capto MMC with its smaller pores has a slower rate of mass transfer than Nuvia cPrime. For both resins, as shown by means of confocal laser scanning miscopy, the adsorption kinetics is controlled by pore diffusion. The main difference is the adsorption kinetics of the mAb, which is the larger of the two proteins studied. Equilibrium binding capacities for lysozyme and a monoclonal antibody (mAb) are also similar for both resins at comparable pH and salt concentrations, although Capto MMC shows a weaker dependence on salt concentration as a result of its more hydrophobic character. Potentiometric titration curves show similar buffering ranges and pK a values for the ligands in both resins and a slightly higher ligand density for Nuvia cPrime. Transmission electron microscopy and inverse size exclusion chromatography indicate a bimodal distribution of pores in Nuvia cPrime, including small pores with 10 nm radius and pores larger than 400 nm, and a monodispersed distribution of pores in Capto MMC, averaging 32 nm in radius. In both resins, the ligand contains a phenyl group, a carboxyl group, and a peptide bond but with a different arrangement. Protein adsorption equilibria and kinetics are obtained experimentally for two multimodal cation exchange resins-Nuvia cPrime, which is based on a polymeric matrix, and Capto MMC, which is based on an agarose matrix.

Capto mmc resin structure